TB-500 Reconstitution Calculator
TB-500 is supplied as a lyophilised powder, so every vial needs reconstituting before anything can be drawn. Enter your vial size, the amount of bacteriostatic water you are adding and your dose, and this calculator converts it into the exact number of units on an insulin syringe — plus your concentration and how many doses the vial holds.
100 units per mL — pick the syringe you have.
Draw to
BAC water
on a U-100 insulin syringe
Drag the syringe to set your dose, or type the values above.
Enter your vial amount and dose, then type the bacteriostatic water or drag the syringe to see the exact units to draw.
Vial sizes and water volumes are shown for convenience — the dose field is deliberately not prefilled and has no suggestions. Enter your own numbers.
Never do this math twice.
The Pep AI app remembers your vials and doses, shows an interactive syringe you draw to, counts down the doses left in each vial, and reminds you when it's time. Free on iOS and Android.
How reconstitution arithmetic works
Three steps, and the calculator does all three. The dose below is an arbitrary figure chosen to demonstrate the arithmetic, not a suggestion:
1. Concentration
Vial amount ÷ water added. A 5 mg vial with 2 mL of water is 2.5 mg/mL.
2. Volume per dose
Your dose ÷ concentration. A 1 mg dose at 2.5 mg/mL is 0.4 mL.
3. Units to draw
Volume per dose × 100 for a U-100 syringe (or × 40 for a U-40). 0.4 mL on a U-100 syringe is 40 units.
Worked example
You have a 5 mg vial, you want a 1 mg dose, and you add 2 mL of bacteriostatic water. Concentration is 5 ÷ 2 = 2.5 mg/mL. Volume per dose is 1 ÷ 2.5 = 0.4 mL. On a U-100 syringe that's 0.4 × 100 = 40 units, and the vial holds 5 doses.
What to know before mixing TB-500
TB-500 is a synthetic peptide based on an active region of thymosin beta-4. It has no approval for human use in any major jurisdiction and is sold labelled for laboratory research, which is also why vial sizes vary between suppliers rather than following a standard presentation.
That variation is exactly why the arithmetic has to be done per vial rather than carried over. A calculation from a previous vial is only valid if both the vial size and the water volume were identical, and neither is guaranteed between orders.
Write the water volume on the vial when you mix it. Concentration cannot be recovered from the vial afterwards, and every unit figure you record depends on it — see the reconstitution guide for the full procedure.
Frequently asked questions
This calculator is provided for informational and educational purposes only and is not medical advice. Always confirm dosing with a qualified healthcare professional and double-check your own measurements before administering anything.
Built by the Pep AI team.